Contamination Free Microalgae Cultures

Firstly, obtain a live starter culture from a reputable source. Sellers usually post "microscope verification" and clean cultures. Purchasing phytoplankton from other sources can introduce unwanted rotifers and bacteria, which is a contamination disaster. Visually, look for clean, vivid cultures.

There are three types of sterilization/disinfectants you need to know about: chemical sterilization, boiling, and filtration. When first receiving your cultures, wipe everything down with hydrogen peroxide or 91% rubbing alcohol. This kills many bacteria on the outside containers. Boiling seawater used to culture your phytoplankton is essential, as saltwater can be a reservoir for invaders. Filtration is needed to cleanly produce F/2 media, the grow solution to cultivate microalgae.

Having a clean workspace is key, a laminar flow hood, still air box, or a DIY culture box is great to prevent spores in the air from settling in your algae culture.

Aeration is key, stagnant water will breed bacteria. Also, turn off any fans to prevent dust from settling.