Contamination Free Microalgae Cultures

Firstly, obtain a live starter culture from a reputable source. Sellers usually post "microscope verification" and clean cultures. Purchasing phytoplankton from other sources can introduce unwanted rotifers and bacteria, which is a contamination disaster. Visually, look for clean, vivid cultures.

There are three types of sterilization/disinfectants you need to know about: chemical sterilization, boiling, and filtration. When first receiving your cultures, wipe everything down with hydrogen peroxide or 91% rubbing alcohol. This kills many bacteria on the outside containers. Boiling seawater used to culture your phytoplankton is essential, as saltwater can be a reservoir for invaders. Filtration is needed to cleanly produce F/2 media, the grow solution to cultivate microalgae.

Having a clean workspace is key, a laminar flow hood, still air box, or a DIY culture box is great to prevent spores in the air from settling in your algae culture.

Aeration is key, stagnant water will breed bacteria. Also, turn off any fans to prevent dust from settling.

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Phyconet is an experimental open‑source project. Results will vary depending on your algae strain, reactor setup, and local conditions. We make no guarantees of specific yields. The AI is a research tool, not a certified safety device. By supporting this campaign, you’re helping us test and improve a prototype that we believe will make algae cultivation easier for everyone.

Limitation of Liability
To the fullest extent permitted by law, Orr Biologicals and its owners shall not be liable for any direct, indirect, incidental, or consequential damages arising from your use of the spirulina cultures, the information on this site, or any products derived from them. This includes, but is not limited to, illness, allergic reactions, or economic losses.

Compliance with Local Laws
Spirulina cultivation and sale may be regulated in your area. It is your responsibility to check with your local health or agricultural authority before buying or growing spirulina. We do not certify that our cultures meet any particular legal standard for food safety.

Please note that the creator of MAIN is a minor operating this project

as an educational and non‑commercial open‑source initiative.

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